{"id":2233,"date":"2022-03-07T13:16:15","date_gmt":"2022-03-07T11:16:15","guid":{"rendered":"https:\/\/biocc.fi\/?page_id=2233"},"modified":"2026-03-03T12:57:59","modified_gmt":"2026-03-03T10:57:59","slug":"laboratoriot-ja-laitteisto","status":"publish","type":"page","link":"https:\/\/biocc.fi\/en\/laboratoriot-ja-laitteisto\/","title":{"rendered":"LABORATORIES & EQUIPMENT"},"content":{"rendered":"

Laboratory of bio and circular economy<\/h3>\n
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Savonia University of Applied Sciences\u2019 laboratory of bio and circular economy boosts companies' research and development work and promotes the utilization of industrial side streams as raw materials for recycled fertilizers and soil conditioners. In the start-up phase, the laboratory will focus on microbial-based biorefining and the development of recycled fertilizers and soil conditioners through cultivation experiments. The laboratory especially serves the local business life.<\/p>\n

Biolaboratory is located in Sytytin building<\/strong>, in Savilahti, Kuopio.<\/p>\n<\/div>\n<\/div><\/div><\/div><\/div><\/div><\/div>

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Laboratory services<\/h3>\n
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The Bio and Circular Economy laboratory's services include biogas studies, biogas production potential studies, soil improvement studies, elemental analysis, ICP-OES, nutrient analyses, contaminant analyses, carbon dioxide analyses, recycled fertilizer studies, cultivation experiments and other analysis services.<\/p>\n

See all of our services below and contact us to find the laboratory services that suit your company's needs.<\/p>\n

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Development of soil improvers and recycled fertilizers<\/span><\/strong><\/h4>\n

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We research and develop soil improvers, recycled fertilizers and growing media from the province's side streams. The center of our research is an arch greenhouse, where testing can be carried out on a pot scale, in different pot size categories. The greenhouse also enables use trials of finished fertilizer products.<\/p>\n

The positions of the pots in the greenhouse can be randomized. Conditions can be monitored in terms of temperature and humidity. Test plants grown in the greenhouse can be collected and analyzed for elements, and analysis of growing media samples\/soil samples is also possible in the laboratory. Through our network, you can easily find a farm partner if you need field-size testing.<\/p>\n

In our laboratory, you can determine the dry matter and moisture content of a soil improver or growing media sample in accordance with the SFS-EN 13040 standard and the plant response in accordance with the SFS-EN 16086-2 standard. pH and electrical conductivity determinations are also possible according to standards SFS-EN 13037 and SFS-EN 13038. In addition to these, the research range includes determinations of bulk density and water retention capacity according to an in-house method.<\/p>\n

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Bioprocess research \u2013 Biogas studies and profitability calculations<\/strong><\/span><\/h4>\n

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The laboratory can test and demonstrate biorefinery technologies with laboratory-scale process equipment. For biogas production, the laboratory has equipment from BPC Instruments, which can be used to carry out biogas production potential tests safely and efficiently. The equipment can be used to determine the biogas and methane production potential of the material or material mixture being tested.<\/p>\n

A biogas batch test refers to a test to which no new feed is added during the test, but the test is carried out to the end with a single batch. Before starting the test, the dry matter and organic matter of the materials being tested are determined in accordance with standard SFS 3800 and the pH is measured in accordance with standard SFS 3021 (1979) or SFS-EN 13037. Biogas and\/or methane production potential tests are carried out in accordance with standard VDI 4630_2016.<\/p>\n

The tests are started using an inoculum, i.e. a bacterial transfer from an already operating biogas plant. The inoculum is tested separately and its effect is subtracted from the final results. Our laboratory is responsible for obtaining high-quality inoculum for experimental activities with expertise.<\/p>\n

The experiments determine the cumulative amount of biogas and methane formed. The data is stored in a database as a function of time as a total gas flow, from which it can be downloaded as an Excel file for further processing and profitability calculations.<\/p>\n

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Bioprocess research \u2013 Biohydrogen research and promotion of the hydrogen economy<\/strong><\/span><\/h4>\n

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We research and develop microbial-based biohydrogen production and at the same time support the formation of a hydrogen economy.<\/p>\n

Microbial-based hydrogen is formed as an intermediate product of the anaerobic, or oxygen-free, decomposition process of organic matter. A special group of bacteria, acetogenetic bacteria, decompose volatile fatty acids and alcohols into carbon dioxide, hydrogen and acetate. To maximize hydrogen production, the bacteria's metabolism is directed to produce fatty acids.<\/p>\n

Dark fermentation has been found to be a suitable technology for renewable biohydrogen production. Suitable feedstock for dark fermentation include organic side streams, such as industrial wastewater and agricultural by-products, it is continuous, does not require light, and its energy consumption is also relatively low.<\/p>\n

The experiments determine the amount of hydrogen formed cumulatively using a volumetric tank and a GA-21 plus gas analyzer. The results can be used to produce profitability calculations for the feeds.<\/p>\n

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Elemental analysis<\/strong><\/span><\/h4>\n

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The laboratory is currently able to analyze 20 elements using an ICP-OES analyzer, the analyses are based on the standard SFS-EN ISO 11885. The element repertoire includes major and minor nutrients important for plant growth, trace nutrients and harmful substances according to the fertilizer product legislation. The ICP-OES equipment can analyze both solid and liquid organic and inorganic samples. Pretreatment of solid samples to liquid form is performed using a microwave wet combustion oven.<\/p>\n

The laboratory can also analyze nitrogen and carbon content (TC\/TN, TOC and TIC) using Vario Cube Max analyzers.<\/p>\n

We can comprehensively analyze various sample matrices, such as industrial side streams, sludge, manure, biomass, plants, rejects, growth media and biochar.<\/p>\n

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Pre-processing of circular economy side streams<\/strong><\/span><\/h4>\n

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To ensure high-quality elemental analysis, test materials can always be reduced and homogenized if necessary.<\/p>\n

The Endecotts Minor 200 vertical screen is suitable for dry screening in the particle size range of 38 \u00b5m \u2013 125 mm. The screen can be used to separate fine-structured materials into different size classes. The vertical screen is suitable for screening various side streams, such as soil and rock materials, fertilizer products, seeds, sand and building materials. The maximum sample input capacity is 3 kg.<\/p>\n

The Retsch PM 100 ball mill is suitable for grinding various organic and inorganic materials, such as soil and plant samples. The PM 100 ball mill is suitable for fine grinding of soft, hard, brittle and fibrous materials. The feed size of the material to be ground should be less than 10 mm and the sample volume is 10\u201335 ml \/ grinding vessel.<\/p>\n

The Labtech 1580R centrifuge is intended for separating different components of samples based on their density. It is suitable, for example, for separating the liquid and solid fractions of rejects, thus enabling the determination of their elemental concentrations in separate fractions.<\/p>\n

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Other analysis services<\/strong><\/span><\/h4>\n

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Other analysis services include the following measurements\/determinations: alkalinity, volatile fatty acids, soluble oxygen, pH, ORP (redox potential), electrical conductivity, dry matter content, organic matter content, moisture and ash content, bulk density, water retention capacity and gas composition measurements.<\/p>\n

In our laboratory you will find modern and contemporary Metler Toledo scales for accurate weighing.<\/p>\n

We process laboratory equipment with a washing and disinfection washer (DEKO 260), which meets the requirements of the standards EN ISO 15883-1 and EN ISO 15883-2.<\/p>\n

The laboratory has water purification equipment for the production of pure water (type 2) and ultrapure water (type 1). Merck's Elix Essential water purification equipment produces ion-exchanged water at 15 l\/h and Merck's Synergy water purification equipment produces ultrapure water at 1.5 l\/min.<\/p>\n<\/div>\n<\/div><\/div><\/div><\/div><\/div><\/div>

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Are you interested in bio and circular economy laboratory services? Contact us!<\/p>\n<\/div>\n<\/div><\/div><\/div><\/div><\/div><\/div>

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Laboratory email<\/h3>\n

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biolaboratorio@savonia.fi<\/a><\/p>\n<\/div>\n<\/div><\/div><\/div><\/div>

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Harri Auvinen<\/h3>\n

R&D manager<\/strong><\/p>\n

harri.auvinen@savonia.fi<\/a><\/p>\n

puh.\u00a0044 785 6923<\/a><\/p>\n<\/div>\n<\/div><\/div><\/div><\/div>

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Eevi Minkkinen<\/h3>\n

Research engineer<\/strong><\/p>\n

eevi.minkkinen@savonia.fi<\/a><\/p>\n

puh.\u00a0044 785 6159<\/a><\/p>\n<\/div>\n<\/div><\/div><\/div><\/div><\/div><\/div>

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Laboratory equipment<\/h3>\n
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BIOGAS AND BIOPROCESS RESEARCH<\/h3>\n
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Biogas batch tests with BioProcessControl equipment<\/h3>\n
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The methane production potential of the studied material or material mixture can be determined with the biogas batch test. A biogas batch test refers to a test in which no new input is added during the test, but the test is carried out with one \"input\" until the end.<\/p>\n

The BPC equipment has 18 reactors with a capacity of 1 liter equipped with mechanical stirrers. The reactors are placed in a water bath, so the experiments can be carried out either in a mesophilic (35-38 \u00b0C) or thermophilic temperature range (55 \u00b0C). The accumulation of biogas and the proportion of methane are measured cumulatively from the reactors.<\/p>\n

The tests are started using bacterial transfer from an already operational biogas plant, the share of the effect of which is reduced in the calculation of the results. Biogas batch tests are done in accordance with method VDI 4630 (2016).<\/p>\n

The hydrogen production potential can also be determined with the equipment in the same way.<\/p>\n

The BioProcessControl equipment stores the data in the database as the total gas flow (Accumulated gas volume Nml) as a function of time.<\/p>\n

The gas flow is recorded by the equipment in every minute, so the data can be viewed on a minute, hour and day level. In addition to the total flow rate, the data is stored as the amount of methane in the same unit.<\/p>\n

Data from the software can be downloaded as an excel file and can be easily processed further.<\/p>\n<\/div>\n<\/div><\/div><\/div><\/div>

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Biogas batch tests with 5l capacity reactors<\/h3>\n
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The methane production potential of the studied material or material mixture can be determined with the biogas batch test. A biogas batch test refers to a test in which no new input is added during the test, but the test is carried out with one \"input\" until the end.<\/p>\n

As second option, the tests in the biolaboratory can be carried out with 5l capacity reactors, where the gas is collected in a separate gas collection bag and analyzed using the volume measuring tank in the picture and the Biogas5000 gas analyzer. The analyzer detects methane, carbon dioxide and oxygen in volume percentages and hydrogen sulfide at the ppm level in the gas mixture.<\/p>\n<\/div>\n<\/div><\/div><\/div><\/div>

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Fermentation equipment<\/h3>\n
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The bio and circular economy laboratory has two fully automated fermentors for microbiological research that can be operated batch or continuous. Conditions can be adjusted to be oxygenated or deoxygenated as needed. The fermentors can be processed in series or in parallel and the reactors have a volume of 5l. The equipment includes mechanical mixing and temperature control from 5 degrees to 70 degrees (water jacket & heating plate). The control unit has integrated 3 separate pumps, which enable e.g. nutrient supply and automatic pH adjustment. The equipment has on-line measurement sensors for pH, ORP and O2<\/em> and data storage in a database. Option for gas collection and collection and analysis of liquid end products.<\/p>\n<\/div>\n<\/div><\/div><\/div><\/div>

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BIOGAS 5000<\/h3>\n
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The BIOGAS 5000 gas meter measures methane (CH4<\/sub>), carbon dioxide (CO2<\/sub>), oxygen (O2<\/sub>) and other gases in volume percentages. Gas meter gives hydrogen sulfide (H2<\/sub>S) as ppm. For reliable biogas analysis, there must be at least 0.5 l of gas. The equipment is intended for field use, so it can be used to perform measurements from other places as well, for example at a landfill.<\/p>\n<\/div>\n<\/div><\/div><\/div><\/div>

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GA-21 PLUS hydrogen analyzer<\/h3>\n
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GA-21 PLUS hydrogen analyzer measures hydrogen and carbon dioxide in volume percentages (0-100%). For a reliable hydrogen measurement, there must be at least half a liter of gas.<\/p>\n<\/div>\n<\/div><\/div><\/div><\/div>

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Pretreatment techniques and analyses for biomasses and side streams<\/h3>\n
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Determination of dry matter and organic matter<\/h3>\n
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Determination of dry matter and organic matter<\/p>\n

An essential part of biogas studies is to find out the amount of dry matter and organic matter in the feed before and after the tests. The determinations are made in the laboratory based on the standard SFS3008 determination of water, sludge and sediment dry matter and annealing residue. Based on the determination, it is possible to evaluate how well the feed is suitable for the biogas process, and based on the TS\/VS reductions, it is possible to evaluate how well the material breaks down in the process. Determinations are made in crucibles brought to standard weight and cooling is done in desiccators.<\/p>\n<\/div>\n<\/div><\/div><\/div><\/div><\/div><\/div>

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WTW Multi 3620 IDS multimeter<\/h3>\n
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The operation of microbiological applications also needs accurate monitoring analytics. The most typical parameters to monitor are pH, conductivity, oxygen and reduction potential. The exact analysis of these is carried out in the biolaboratory with WTW's portable multimeter, which is suitable for both field and laboratory work.<\/p>\n<\/div>\n<\/div><\/div><\/div><\/div><\/div><\/div>

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Level shaker<\/h3>\n
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Edmund B\u00fchler's SM 30 C Control level shaker can be used to pre-treat laboratory samples. The level shaker also enables pH determinations in accordance with the standard SFS-EN 13037 \"Soil conditioners and growing platform determination of pH value\" and electrical conductivity determinations in accordance with the SFS-EN 13038 \"Soil conditioners and growing platform determination of electrical conductivity\" standard. Determinations according to the standard are used e.g. soil conditioner and recycled fertilizer in research work and in the determination of the pH of feed materials for biogas tests.<\/p>\n<\/div>\n<\/div><\/div><\/div><\/div><\/div><\/div>

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Automatic titrator for determining fatty acids and alkalinity<\/h3>\n
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The operation of microbiological applications needs accurate monitoring analytics. The most typical parameters that are monitored in the production of both biohydrogen and biogas are the fatty acids and alkalinity formed in the metabolism of microbes.<\/p>\n

Alkalinity describes the ability of the process to resist a change in pH and is caused by hydroxides, carbonates and hydrogen carbonates, as well as silicates, phosphates, borates, arsenates and aluminates. Alkalinity describes the buffer capacity of the reactor\/process and is expressed as mg CaCO3<\/sub>\/l or mmol\/l. In a biogas plant, the recommended level is between 3500\u20135000 mg CaCO3<\/sub>\/l.<\/p>\n

By determining the concentration of volatile fatty acids, i.e. VFA (Volatile Fatty Acids), the functionality of the bioprocess can be verified. The determination is based on boiling the sample in acid so that (Bi) carbonates break down and carbon dioxide is removed. A functioning bioprocess depends on the balance between the bacteria present in it. If this balance is disturbed, the concentration of volatile fatty acids produced by acid-forming bacteria rises, dropping the pH to a dangerously low level. The determination of VFA can therefore determine the optimal level of fatty acids and guide the microbiological process in the right direction.<\/p>\n<\/div>\n<\/div><\/div><\/div><\/div><\/div><\/div>

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Heating cabinets and annealing furnace<\/h3>\n
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The laboratory uses four heating cabinets\/incubators and one annealing furnace. These enable controlled temperature conditions and can be used in the laboratory for sample drying, sterilization and other pretreatment. In the laboratory, the most typical applications of heating cabinets are biogas input experiments and drying of plant samples from cultivation experiments before analysis.<\/p>\n<\/div>\n<\/div><\/div><\/div><\/div><\/div><\/div>

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Sieve shaker \/ Endecotts Minor 200<\/h3>\n
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Endecotts Minor 200 sieve shaker is suitable for dry screening in the particle size range of 38 \u00b5m - 125 mm. The sieve shaker can be used to separate fine-structured materials into different size classes. The device is suitable for screening various side streams, soil and rock materials, fertilizer products, seeds, sand and construction materials. The maximum sample input capacity is 3 kg.<\/p>\n<\/div>\n<\/div><\/div><\/div><\/div><\/div><\/div>

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Planetary Ball Mill \/ Retsch PM 100<\/h3>\n
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Retsch PM 100 planetary ball mill can be used to grind various organic and inorganic materials such as soil and plant samples, biochar and sand. The PM 100 ball mill is suitable for fine grinding of soft, hard, brittle and fibrous materials. The feed size of the material to be ground should be less than 10 mm and the sample volume is 10-35ml \/ grinding vessel.<\/p>\n<\/div>\n<\/div><\/div><\/div><\/div><\/div><\/div>

Scales and weighing capacities<\/h3>\n
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Mettler Toledo XS204<\/h3>\n
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max 220 g (d = 0,1 mg), weighing with 4 decimals<\/p>\n

Analytical scale<\/em><\/p>\n<\/div>\n<\/div><\/div><\/div><\/div>

Mettler Toledo XSR205DU<\/h3>\n
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max 220 g (d = 0,01 mg \/ 0,1 mg), weighing with 5 decimals<\/p>\n

Analytical scale<\/em><\/p>\n<\/div>\n<\/div><\/div><\/div><\/div>

Mettler Toledo MR6002<\/h3>\n
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max 6200 g (d = 0,01 g)<\/p>\n

(Upper cup scale \/ precision scale)<\/em><\/p>\n<\/div>\n<\/div><\/div><\/div><\/div><\/div><\/div>

Multi-element analysis<\/h3>\n
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ICP-OES - Inductively Coupled Plasma Optical Emission Spectroscopy<\/h3>\n
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ICP-OES enables the determination of up to more than 70 elements. In ICP-OES technology, the measurement range is typically wide: the ratio of the concentrations of the lowest and the highest element measured at the same time can be 1:1,000 or even 1:10,000. Thus, the need for sample dilutions is minimal.<\/p>\n

Samples can be measured as:<\/p>\n

1. Qualitative measurement \u2013 qualitative measurement of the elements contained in an unknown sample.
\n2. Semi-Quantitative measurement - quantitative measurement - the device estimates the concentrations of elements with 10% certainty.
\n3. Quantitative measurement \u2013 accurate quantitative determination of elemental concentrations. Unknown samples are compared to known measured samples<\/p>\n

For ICP-OES analysis, the samples must be treated as liquid form, which can be done with a microwave acid digestion.<\/p>\n<\/div>\n<\/div><\/div><\/div><\/div><\/div><\/div>

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Microwave Acid Digestion<\/h3>\n
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Decomposing solid samples into liquid form is done by dissolving the sample to be analyzed with a suitable solvent. The solvent is chosen so that the sample decomposes almost completely, the non-degraded parts can be filtered out of the sample.<\/p>\n

In microwave acid digestion, the sample is exposed to a strong acid solution in a closed container. The temperature and pressure of the container is increased by using microwaves. The high pressure prevents the sample from boiling and contamination. The increase in temperature and pressure accelerates thermal decomposition and the dissolution of heavy metals.<\/p>\n

In the biolaboratory, the samples are decomposed either with nitric acid or aqua regia in a 24-place sample container. Typically, the amount of sample required for acid digestion is 5 g.<\/p>\n<\/div>\n<\/div><\/div><\/div><\/div><\/div><\/div>

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Elementar vario MAX cube<\/h3>\n
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Elementar's vario MAX cube analyzer can analyze total nitrogen (TN) and total carbon (TC) as well as organic carbon (TOC) and inorganic carbon (TIC) using a different program.<\/p>\n

Elementar has several methods for different sample matrices. The method is chosen according to the moisture or organic matter contained in the matrix. Solid samples can be analyzed with the device. Samples do not need to be dried before analysis, so the device is also suitable for the analysis of samples containing moisture.<\/p>\n

The device has a 90 crucible autosampler. The samples are analyzed in a steel crucible and in an incinerator. The incinerator consists of three vertical columns: combustion, post-combustion and reduction column. Post-combustion is needed to ensure complete combustion of the sample. The device has automatic ash and water removal. Helium is used as carrier gas.<\/p>\n<\/div>\n<\/div><\/div><\/div><\/div><\/div><\/div>

SOIL CONDITIONERS AND RECYCLED FERTILIZERS<\/h3>\n
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Arch greenhouse (18 m2)<\/h3>\n
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The greenhouse enables research and development of recycled fertilizers and soil conditioners based on underutilized side streams. The greenhouse can hold approximately 100-150 submersible pots (\u00d8 25 cm) with a soil capacity of 6 liters and a water storage capacity of 2 liters at a time. The order of the pots can be randomized and typically the test matrices are repeated in 3-4 parallel pots. Testing different growth ground is also possible. The experimental layout of cultivation experiments can be implemented to correspond to the amounts of sowing, fertilizer and liming used in agriculture by relating these to the pot scale.<\/p>\n<\/div>\n<\/div><\/div><\/div><\/div>

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EQUIPMENT MAINTENANCE<\/h3>\n
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DEKO 260 washing and disinfecting machine<\/h3>\n
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High-quality equipment maintenance in the bio and circular economy laboratory is provided by the DEKO 260 washing and disinfecting machine, which meets the requirements of standards EN ISO 15883-1 and EN ISO 15883-2. The washing machine enables efficient and fast washing and disinfection programs for the needs of the laboratory, while offering a high overall washing capacity. The washing machine is suitable for various laboratory containers, from a 10 liter plastic bucket to a 20 ml measuring bottle.<\/p>\n<\/div>\n<\/div><\/div><\/div><\/div><\/div><\/div>

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Merck's Elix Essential and Synergy water purification systems<\/h3>\n
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Merck's Elix Essential water purification system and a 60-liter tank for ion-exchanged water. Elix Essential produces ion-exchanged water (15 l\/h), i.e. pure water (type 2) for laboratory needs. Ion-exchanged water is used e.g. in general laboratory applications, such as rinsing electrodes, diluting samples, preparing solutions, and as input for ultrapure water (type 1) water purification system (Synergy).<\/p>\n

Ion-exchanged water is stored in a 60-liter polyethylene tank, which is designed to minimize possible contamination risks during water storage. It is also possible to lead ion-exchanged water from the water tank to the laboratory's washing machine for final rinsing of dishes.<\/p>\n

The Merck Synergy water purification system produces ultrapure water (1.5 l\/min) for laboratory needs. High-quality ultrapure water (type 1) is used in the most critical applications and advanced analytical methods, such as ICP-OES equipment.<\/p>\n<\/div>\n<\/div><\/div><\/div><\/div><\/div><\/div><\/div>","protected":false},"excerpt":{"rendered":"

Savonian bio- ja kiertotalouden laboratoriossa vauhditetaan yritysten tutkimus- ja kehitysty\u00f6t\u00e4 sek\u00e4 edist\u00e4\u00e4n teollisuuden sivuvirtojen hy\u00f6dynt\u00e4mist\u00e4 mm. kierr\u00e4tyslannoitteiden ja maanparannusaineiden raaka-aineina. K\u00e4ynnistysvaiheessa laboratoriossa […]<\/p>\n","protected":false},"author":9,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"page-templates\/page_front-page.php","meta":{"footnotes":""},"class_list":["post-2233","page","type-page","status-publish","hentry"],"yoast_head":"\nLABORATORIOT & LAITTEISTO - Bio and Circular Cluster - North Savo<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/biocc.fi\/en\/laboratoriot-ja-laitteisto\/\" \/>\n<meta property=\"og:locale\" content=\"en_US\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"LABORATORIOT & LAITTEISTO - Bio and Circular Cluster - North Savo\" \/>\n<meta property=\"og:description\" content=\"Savonian bio- ja kiertotalouden laboratoriossa vauhditetaan yritysten tutkimus- ja kehitysty\u00f6t\u00e4 sek\u00e4 edist\u00e4\u00e4n teollisuuden sivuvirtojen hy\u00f6dynt\u00e4mist\u00e4 mm. kierr\u00e4tyslannoitteiden ja maanparannusaineiden raaka-aineina. 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